Real-time blood velocity measurements in human retinal vein using the laser speckle phenomenon.
نویسندگان
چکیده
PURPOSE To measure the in vivo blood velocity in human retinal veins using a laser speckle system. METHODS The system consists of a fundus camera, a diode laser, an image sensor, and a personal computer system. The fundus area, including a target retinal vein, is illuminated with a diode laser through a fundus camera and the laser speckle pattern is imaged onto the area sensor. From the time change of the contrast of the speckle pattern, the normalized blur (NB) value, a quantitative index of blood velocity, was calculated using a logic board. RESULTS In an in vitro experiment, the NB obtained from blood flow in 50-300 microm internal diameter glass capillary tubes, used as an analogue of a retinal vein, correlated with the diameter of the tube, the actual blood flow rate, and the background NB value, which was used as an analogue of choroidal circulation. In the in vivo experiment, the blood velocity in human retinal veins of approximately 50 microm in diameter was estimated in 16 normal human eyes using nomograms based on the result of the in vitro experiment. Velocity averaged 11.1+/-0.6 mm/s (mean +/- SD, n = 16) in retinal veins 53+/-6 microm in diameter. The coefficient of reproducibility of 5-minute interval measurements was 2.5+/-0.9%, and it took 63+/-15 seconds for one measurement. CONCLUSIONS The present methodology is clinically valid for measuring blood velocity in retinal veins.
منابع مشابه
Real-time measurement of human optic nerve head and choroid circulation, using the laser speckle phenomenon.
A modification of a previously described instrument that used the laser speckle phenomenon for noncontact two-dimensional analysis of the fundus tissue circulation was devised so that tissue circulation in the optic nerve head (ONH) or choroid of the human eye could be measured on a real-time basis. The fundus was illuminated by a diode laser spot and the image speckle was recognized by an area...
متن کاملSimultaneous measurement of water flow velocity with fluorescent and speckle imaging technique
The average velocity of water flow has been simultaneously measured with fluorescent and speckle imaging methods. The measured velocities with two methods are in good agreement with each other and it confirms that the speckle imaging method can be used as a confident method to measure the velocity of water flow in a dry leaf. Also the velocity of water flow through thick and thin xylems of a le...
متن کاملLaser Doppler, speckle and related techniques for blood perfusion mapping and imaging.
Laser Doppler velocimetry uses the frequency shift produced by the Doppler effect to measure velocity. It can be used to monitor blood flow or other tissue movement in the body. Laser speckle is a random interference effect that gives a grainy appearance to objects illuminated by laser light. If the object consists of individual moving scatterers (such as blood cells), the speckle pattern fluct...
متن کاملEffect of topical latanoprost-timolol combined therapy on retinal blood flow and circulation of optic nerve head tissue in cynomolgus monkeys.
PURPOSE To evaluate the effects of topical latanoprost and timolol in combined therapy on retinal blood flow and tissue circulation in the optic nerve head (ONH) of the cynomolgus monkey. METHODS Latanoprost (30 microL, 0.005%) was instilled once daily and timolol (30 microL, 0.5%) twice daily for 6 days into 1 eye, and physiological saline into the other eye to serve as control. Blood veloci...
متن کاملLaser speckle contrast analysis (LASCA): a nonscanning, full-field technique for monitoring capillary blood flow.
A new noninvasive technique for monitoring capillary blood flow has been developed. Based on the phenomenon of time-varying laser speckle, it is a digital version of single-exposure speckle photography. It provides a velocity map of the area of interest in real time without the need for scanning. The results of some initial experiments on volunteers are presented.
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Japanese journal of ophthalmology
دوره 43 3 شماره
صفحات -
تاریخ انتشار 1999